Journal: Cureus

Article Title: Role of Facial Nerve Reconstruction With Anastomosis and Polyglycolic Acid Tube in Accelerating Functional Recovery After Axotomy in the Rat Facial Nucleus

doi: 10.7759/cureus.57326

Figure Lengend Snippet: (A, B) Cross-sections of facial nerves immunostained with MBP (green), β3-tubulin (red), and DAPI (blue). Scale bar: 10 μm (A), magnified view. Scale bar: 100 μm (B). (C) Number of myelinated fibers per area relative to the post-operative date. Data are expressed as mean ± standard error of the mean. N = 4. *p < 0.05; **p < 0.01 as determined based on analysis of variance and post-hoc Tukey’s test. DAPI, 4′,6-diamidino-2-phenylindole; MBP, myelin basic protein

Article Snippet: Histology To evaluate axonal degeneration and demyelination, we processed the 14-μm sections for immunostaining using antibodies against myelin basic protein (MBP) (1:400; Merck Millipore, Burlington, MA, USA) and β3-tubulin (1:400; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: